Evaluation and implementation of high throughput PCR-based method for diagnosis and measurement of P. falciparum parasitaemia in clinical trials
TA.2005.40200.006
EDCTP1
Senior Fellowship (SF)
To evaluate a number of different quantitative real-time PCR (qPCR) methods to determine and establish a suitable protocol for routine application in malaria diagnosis and measurement of parasite density. The objectives were: • To establish the application of qPCR determination of malaria parasitaemia for clinical trials • To evaluate parasite density estimates obtained from qPCR amplification of parasite DNA in blood sample for agreement with blood film slide microscopy • To conduct a cost comparison of qPCR with slide microcopy for the determination of malaria parasitaemia.
Point of care diagnostics
Department | Institution | Country |
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Medical Research Council (MRC) Laboratories | Gambia |