Bunjun, R. Riou, C. Soares, A.P. Thawer, N. Müller, T.L. Kiravu, A. Ginbot, Z. Oni, T. Goliath, R. Kalsdorf, B. Von Groote-Bidlingmaier, F. Hanekom, W. Walzl, G. Wilkinson, R.J. Burgers, W.A.

Journal of Infectious Diseases

2017-01-01

Chege, G.K. Burgers, W.A. Stutz, H. Meyers, A.E. Chapman, R. Kiravu, A. Bunjun, R. Shephard, E.G. Jacobs Jr., W.R. Rybicki, E.P. Williamson, A.-L.

Journal of Virology

2013-01-01

Riou, C. Bunjun, R. Müller, T.L. Kiravu, A. Ginbot, Z. Oni, T. Goliath, R. Wilkinson, R.J. Burgers, W.A.

Tuberculosis

2016-01-01

Mohau S. Makatsa F. Millicent A. Omondi Rubina Bunjun Robert J. Wilkinson Catherine Riou Wendy A. Burgers

Frontiers in Immunology

2018-01-01

Burgers, W.A. Ginbot, Z. Shen, Y.-J. Chege, G.K. Soares, A.P. Müller, T.L. Bunjun, R. Kiravu, A. Munyanduki, H. Douglass, N. Williamson, A.-L.

Journal of General Virology

2014-01-01

Frontiers in Immunology

HIV-1 increases susceptibility to pulmonary infection and disease, suggesting pathogenesis in the lung. However, the lung immune environment during HIV infection remains poorly characterized. This study examined T cell activation and the cytokine milieu in paired bronchoalveolar lavage (BAL) and blood from 36 HIV-uninfected and 32 HIV-infected participants. Concentrations of 27 cytokines were measured by Luminex, and T cells were phenotyped by flow cytometry. Blood and BAL had distinct cytokine profiles (p=0.001). In plasma, concentrations of inflammatory cytokines like IFN-γ (p=0.004) and TNF-α (p=0.004) were elevated during HIV infection, as expected. Conversely, BAL cytokine concentrations were similar in HIV-infected and uninfected individuals, despite high BAL viral loads (VL; median 48,000 copies/ml epithelial lining fluid). HIV-infected individuals had greater numbers of T cells in BAL compared to uninfected individuals (p=0.007); and BAL VL positively associated with CD4+ and CD8+ T cell numbers (p=0.006 and p=0.0002, respectively) and CXCL10 concentrations (p=0.02). BAL T cells were highly activated in HIV-infected individuals, with nearly 2-3 fold greater frequencies of CD4+CD38+ (1.8-fold; p=0.007), CD4+CD38+HLA-DR+ (1.9-fold; p=0.0006), CD8+CD38+ (2.8-fold; p=0.0006), CD8+HLA-DR+ (2-fold; p=0.022) and CD8+CD38+HLA-DR+ (3.6-fold; p<0.0001) cells compared to HIV-uninfected individuals. Overall, this study demonstrates a clear disruption of the pulmonary immune environment during HIV infection, with readily detectable virus and activated T lymphocytes, which may be driven to accumulate by local chemokines.

2021-06-30

Bunjun R Omondi F Makatsa M Keeton R Milimu JW Muller T Prentice C Wilkinson RJ Riou C Burgers W

Journal of Immunology

2021-08-13

Rubina Bunjun Fidilia M.A. Omondi Mohau S. Makatsa Tracey L. Müller Caryn S.L. Prentice Robert J. Wilkinson Catherine Riou Wendy A. Burgers

2019-08-13