Bruno SENGHOR Eglantine Mathieu-Begné Olivier Rey Souleymane Doucouré Doudou Sow Bocar Diop Mariama Sène Jérôme Boissier Cheikh Sokhna

2022-04-22

New Microbes New Infect

We report the main characteristics of 'Bacillus dakarensis' P3515(T) sp. nov., 'Bacillus sinesaloumensis' P3516(T) sp. nov., 'Gracilibacillus timonensis' P2481(T) sp. nov., 'Halobacillus massiliensis' P3554(T) sp. nov., 'Lentibacillus massiliensis' P3089(T) sp. nov., 'Oceanobacillus senegalensis' P3587(T) sp. nov., 'Oceanobacillus timonensis' P3532(T) sp. nov., 'Virgibacillus dakarensis' P3469(T) sp. nov. and 'Virgibacillus marseillensis' P3610(T) sp. nov., that were isolated in 2016 from salty stool samples (\textgreater/=1.7% NaCl) from healthy Senegalese living at Dielmo and N'diop, two villages in Senegal.

2017-02-01

2020-07-01

Bruno Senghor

Parasit Vectors

Background: Global changes are reshaping the distribution of vector-borne diseases by spreading vectors to previously non-endemic areas. Since 2013, urogenital schistosomiasis has emerged in Corsica and threatens European countries. Gastropod vectors release schistosome larvae that can infect humans who come into contact with freshwater bodies. Monitoring schistosomiasis host vectors is a prerequisite to understand and subsequently to control this pathogen transmission. Because malacological surveys are time consuming and require special expertise, the use of a simple molecular method is desirable.

Methods: The aim of this study is to develop a ready-to-use protocol using the LAMP (loop-mediated isothermal amplification) method to detect environmental DNA of Bulinus truncatus, vector of Schistosoma haematobium. Interestingly, LAMP method possesses all the characteristics required for adaptability to field conditions particularly in low-income countries: speed, simplicity, lyophilized reagents, low cost and robustness against DNA amplification inhibitors. We have tested this new method on Corsican water samples previously analysed by qPCR and ddPCR.

Results: We demonstrate that our diagnostic tool B. truncatus eLAMP (Bt-eLAMP) can detect the eDNA of Bulinus truncatus as effectively as the two other methods. Bt-eLAMP can even detect 1/4 of positive samples not detectable by qPCR. Moreover, the complete Bt-eLAMP protocol (sampling, sample pre-process, amplification and revelation) does not require sophisticated equipment and can be done in 1 ½ h.

Conclusions: LAMP detection of environmental DNA provides large-scale sensitive surveillance of urogenital schistosomiasis possible by identifying potentially threatened areas. More generally, eLAMP method has great potential in vector-borne diseases and ecology.

2023-02-28

Senghor B Diallo A Sylla SN Doucouré S Ndiath MO Gaayeb L Djuikwo-Teukeng FF Bâ CT Sokhna C

2014-01-01

Senghor B Diaw OT Doucoure S Sylla SN Seye M Talla I Bâ CT Diallo A Sokhna C

2015-01-01

New Microbes and New Infections

2017-11-01

Senghor B Diaw OT Doucoure S Seye M Talla I Diallo A Bâ CT Sokhna C

2015-01-01

Papa Mouhamadou Gaye Souleymane Doucoure Bruno Senghor Babacar Faye Ndiaw Goumballa Mbacké Sembène Coralie L’Ollivier Philippe Parola Stéphane Ranque Doudou Sow Cheikh Sokhna

Pathogens

2021-07-08

2020-11-01

Bruno SENHGOR

Current Research in Parasitology & Vector-Borne Diseases (CRPVBD)

Bulinus senegalensis and Bulinus umbilicatus, two sympatric freshwater snails found in temporal ponds in Senegal, were thought to be involved in the transmission of Schistosoma haematobium and/or Schistosoma curassoni. To better understand the role of these Bulinus species in the transmission of human and animal Schistosoma species, B. senegalensis and B. umbilicatus were collected in 2015, during a malacological survey, from a temporal pond in Niakhar, central Senegal. Snails were induced to shed cercariae on two consecutive days. Individual cercariae from each snail were collected and preserved for molecular identification. Infected snails were identified by analysis of a partial region of the cytochrome c oxidase subunit 1 (cox1) gene. Six individual cercariae shed from each infected snail were identified by analyses of the cox1, nuclear ITS and partial 18S rDNA regions. Of the 98 snails collected, one B. senegalensis had a mixed infection shedding S. haematobium, S. bovis and S. haematobium-S. bovis hybrid cercariae and one B. umbilicatus was found to be shedding only S. haematobium. These data provide molecular confirmation for B. senegalensis transmitting S. bovis and S. haematobium-S. bovis hybrids in Senegal. The multiple Bulinus species involved in the human urogenital schistosomiasis in Senegal provides a high force of transmission warranting detailed mapping, surveillance and regular treatment of at-risk populations.

2023-02-01

Bruno Senghor Cheikh Sokhna Raymond Ruimy Jean-Christophe Lagier

Human Microbiome Journal

2018-02-01

Bruno Senghor

Current Research in Parasitology & Vector-Borne Diseases

ulinus senegalensis and Bulinus umbilicatus, two sympatric freshwater snails found in temporal ponds in Senegal, were thought to be involved in the transmission of Schistosoma haematobium and/or Schistosoma curassoni. To better understand the role of these Bulinus species in the transmission of human and animal Schistosoma species, B. senegalensis and B. umbilicatus were collected in 2015, during a malacological survey, from a temporal pond in Niakhar, central Senegal. Snails were induced to shed cercariae on two consecutive days. Individual cercariae from each snail were collected and preserved for molecular identification. Infected snails were identified by analysis of a partial region of the cytochrome c oxidase subunit 1 (cox1) gene. Six individual cercariae shed from each infected snail were identified by analyses of the cox1, nuclear ITS and partial 18S rDNA regions. Of the 98 snails collected, one B. senegalensis had a mixed infection shedding S. haematobium, S. bovis and S. haematobium-S. bovis hybrid cercariae and one B. umbilicatus was found to be shedding only S. haematobium. These data provide molecular confirmation for B. senegalensis transmitting S. bovis and S. haematobium-S. bovis hybrids in Senegal. The multiple Bulinus species involved in the human urogenital schistosomiasis in Senegal provides a high force of transmission warranting detailed mapping, surveillance and regular treatment of at-risk populations.

2023-01-25

Senghor B Diaw OT Doucoure S Seye M Diallo A Talla I Bâ CT Sokhna C

2016-03-01

Zein-Eddine R Djuikwo-Teukeng FF Al-Jawhari M Senghor B Huyse T Dreyfuss G

2014-01-01

September 2018International journal of obesity

2018-09-01

Antonie van Leeuwenhoek volume

2019-01-01

Antonie Van Leeuwenhoek International = Journal of General and Molecular Microbiology, 111 (7), 1225-1236. ISSN 0003-6072

2018-01-01