Project Title

TMA2017 PF Abubakar Shaaban

EDCTP Project Call

EDCTP-AREF Preparatory Fellowships (PF)

Project Objectives

To determine the utility of whole-genome sequencing in predicting for drug resistance genotypes, identifying and characterizing adaptive mutations in the evolved genotypes, and approaches to study the genetic constraints on the evolution of resistance amongst M. Tuberculosis, and S. aureus isolates in Tanzania. Specifically the study aims: i. To isolate M. Tuberculosis and S. aureus isolates from TB presumptive patients attending peripheral TB diagnostic centres in Dar es Salaam, Tanzania. ii. To analyse M. Tuberculosis complex genotypes and to characterize adaptive mutations and predict their drug resistance profiles using Next Generation Sequencing. iii. To analyse S. aureus isolates genotypes and to characterize their adaptive mutations and predict their resistance profiles by using Next Generation Sequencing. iv. To investigate on the utility of Next Generation Sequencing as a routine diagnostic tool for prediction of drug resistant isolates in TB control programmes in resource limited settings of Tanzania.

Study Design

A prospective population-based surveillance will be conducted in three urban hospitals in Dar es Salaam, Tanzania namely, Mwanayamala Hospital in Kinondoni district, Amana Hospital in Ilala district and Temeke Hospital in Temeke district.

Host Organisation

Current Organisation

Current Job Title

Biography