EDCTP Alumni Network

Fostering excellence and collaboration in the next generation of researchers

Call EDCTP Clinical Research & Development Fellowship (R&D F)
Programme EDCTP2
Start Date 2019-02-01
End Date 2021-07-31
Project Code TMA2017IF-1997
Status Active


European Vaccine Initiative, Germany

Host Organisation

Institution Country
Institute of Human Virology Nigeria LTDGTE (IHVN) Nigeria

Current Organisation

Institute of Human Virology Nigeria

Current Job Title

Senior Research Officer

Students Supervised

Type Name Title University Start Date End Date


Role Committee/board Start Date End Date


Institution Degree Year
University of the Western Cape, South Africa Ph.D. (Biotechnology) 2013-03-15
University of the Western Cape, South Africa BSc. Honours (Biotechnology) 2003-03-26
University of Nigeria, Nigeria BSc. Honours (Microbiology) 1999-12-31

Areas Of Specialisation

Human Immuno-deficiency Virus (HIV) Sequencing of HIV to understand molecular basis for failure and for typing Recombinant protein expression and purification Structure and Function of Proteins Molecular Biology



1. Ezenwa James Onyemata, Emmanuel Jonathan, Olasinbo Balogun, Ndidi Agala, Petronilla Jean Ozumba, Talishiea Croxton, Sunji Nadoma, ThankGod Anazodo, Sam Peters, Christian M. Beiswanger, Alash'le Abimiku
Analytical Biochemistry Volume 614, 1 February 2021, 114023

Background: The growth in genomic medicine has resulted in increased understanding of


topical areas in disease, discovery, pathogenesis and evolutionary biology. Fundamental to

data acquisition in genomic science is the quality of Deoxyribonucleic wAcid (DNA). DNA

is qualified using methods which may be expensive for low to middle income country

settings (LMICs) where cost is a constraint.

Method: We evaluated spectrophotometry, spectrofluorometry, agarose gel electrophoresis

and double restriction digestion using HindIII and EcoRI restriction enzymes with the

objective of identifying the most cost effective method(s) in an LMIC setting of Nigeria.

NanoDrop provided the lowest cost per sample at $0.97 followed by agarose gel

electrophoresis at $1.63, double restriction digestion plus agarose gel electrophoresis at

$2.216 and Qubit Fluorometer at $5.226. NanoDrop provides information on sample purity

and concentration, whereas agarose gel electrophoresis provides information on sample

integrity. Qubit Fluorometer provides the absolute concentration of double stranded DNA,

while double restriction digestion reactions provides information on the presence inhibiting


Results: Our results show that NanoDrop and agarose gel electrophoresis at a minimum is

ideal for assessing the quality of DNA as purity, integrity and quantity can be determined

without the use of the more expensive fluorescence method.



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