EDCTP Alumni Network

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All Profiles
Call Senior Fellowship (SF)
Programme EDCTP1
Start Date 2011-03-10
End Date 2013-03-09
Project Code TA.2010.40200.016
Status Completed

Title

Pre-clinical evaluation of dendritic cell targeted consensus B, C, CRFO2_AG and MOSAIC HIV gag protein vaccines in PBMC from chronically infected patients in Central Africa

Objectives

To examine if a DC targeted consensus B HIV gag p24 protein vaccine could recall in vitro pre-existing gag specific T cells in PBMCs of subjects chronically infected with unrelated HIV-1 strains prevalent in Africa To compare in terms of magnitude, breadth, and depth T cell responses recalled in vitro in PBMCs of subjects chronically infected with HIV-1 in central Africa by 4 four different DC targeted HIV gag p24 protein vaccines based on CRFO2_AG, C, B and MOSAIC HIV gag sequences, which are designed to address the problems associated with HIV-1 diversity.

Host Organisation

Institution Country
CIRCB Cameroon
CIRCB Cameroon
CIRCB Cameroon

Participants

Name Institution Country
Vittorio Colizzi International Reference Centre Chantal Biya (CIRCB) Cameroon
Ralph Steinman The Rockerfeller University United States
Klaus Uerberla Ruhr University Bochum Germany

Study Design

Laboratory pre-clinical evaluation of dendritic cell antigens and HIV gag protein vaccines

Students Supervised

Type Name Title University Start Date End Date
Msc Nji Nadesh: Ms faculty of health sciences of the university Buea, Cameroon 2010 2012
Msc MENGUE NDJANA Sonia Nina Ms the department of Biomedical sciences of the University of Ngoundere, Cameroon 2016 2018
Msc TAKOUA DOLINE Ms the department of Biomedical sciences of the University of Ngoundere, Cameroon 2016 2018
Msc Fangnang Ndeyong Tatiana Ms The department of biochemistry of the Yaounde University 1, Cameroon 2012 2014
Msc clavair mbai-oulam Mr The department of biochemistry of the Yaounde University 1, Cameroon 2012 2014
Msc Ghislain Donald Njambe Priso Mr the department of animal biology and physiology of the Yaounde University 1, Cameroon 2012 2014

Results & Outcomes

The project contributed to training of 4 PhD students, 2 MSc and 1 nurse. The EDCTP funds have been used to purchase a number of equipment. The project has established links with University of Yaounde 1, Institut Pasteur Cameroon, Case Western Reserve University and Tromsø Science Park of Norway.

Publications

Maamary J, Array F, Gao Q, García-Sastre A, Steinman RM, Palese P and Nchinda G. Newcastle disease virus expressing a dendritic cell targeted HIV gag protein induces a potent gag specific immune response in mice. J. Virol. March 2011 vol. 85 no. 5 2235-2246
Alba Grifoni et al. Global Assessment of Dengue virus-specific CD4+ T cell responses in Dengue-Endemic. Frontiers in Immunology volume 8 Article 1309
Ngu LN et al. Dendritic cell targeted HIV-1 gag protein vaccine provides help to a recombinant Newcastle disease virus vectored vaccine including mobilization of protective CD8+ T cells. Immun Inflamm Dis. 2017 Dec 4. doi: 10.1002/iid3.209
Alain Bopda Waffo et al. Surface Engineering of Recombinant RNA Coliphage Qβ to Display gp41 Membrane-Proximal External-Region Epitopes from HIV-1. Journal of clinical and experimental. December 2017 DOI10.1038/nprot.2012.085
Carole, S et al.The effect of antiretroviral naïve HIV-1 infection on the ability of Natural Killer cells to produce IFN-γ upon exposure to plasmodium falciparum -infected erythrocytes. DOI: 10.1159/000467386
Ngu, L et al. In vivo targeting of protein antigens to dendritic cells using anti-DEC-205 single chain antibody improves HIV Gag specific CD4+ T cell responses protecting from airway challenge with recombinant vaccinia-gag virus. Immun Inflamm Dis. doi: 10.1002/iid3.151

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