Dr Michael Owusu
| Call | Career Development Fellowship (CDF) |
| Programme | EDCTP2 |
| Start Date | 2017-06-01 |
| End Date | 2020-05-31 |
| Project Code | TMA2015CDF1033 |
| Status | Completed |
Title
Nasopharyngeal and Lung Microbiota in HIV Positive Children Presenting with Respiratory Disease
Objectives
1.Characterise the microbiota of the nasopharyngeal and lung niche of HIV positive and negative children 2. Correlate specific microbiota with the respiratory disease and outcomes in HIV infected and non-infected children. 3.Determine the risk factors associated with exposure to specific microbiota.
Host Organisation
| Institution | Country |
|---|---|
| Kumasi Centre for Collaborative Research in Tropical Medicine (KCCR) | Ghana |
Participants
| Name | Institution | Country |
|---|---|---|
| Prof Hubert Endz | Fondation Merieux | France |
Study Design
Mixed case-control and longitudinal study design
Sites
| Komfo Anokye Teaching Hospital |
Phd Study
| Title | University | Start Date | End Date |
|---|---|---|---|
| Human Coronaviruses associated with respiratory tract infections in children | Kwame Nkrumah University of Science and Technology | 2011-09-01 | 2014-07-01 |
Students Supervised
| Type | Name | Title | University | Start Date | End Date |
|---|---|---|---|---|---|
| FWACP | Lotenna Elsie Kalu | Dr | West African College of Physicians and Surgeons | 2017 | 2019 |
| MSc | Eric Adu | Mr | Kwame Nkrumah University of Science and Technology | 2018 | 2020 |
| MSc | Eugene Adu | Mr | Kwame Nkrumah University of Science and Technology | 2018 | 2020 |
Publications
| Trop. Med. Infect. Dis |
| Trop. Med. Infect. Dis |
Current Organisation
Kwame Nkrumah University of Science and Technology (KNUST)
Current Job Title
Post-doctoral Research Scientist
Students Supervised
| Type | Name | Title | University | Start Date | End Date |
|---|---|---|---|---|---|
| Master's student | Eric Adu | Mr | Kwame Nkrumah University of Science and Technology | 2018 | 2020 |
| Fellowship | Lotenna Kalu | Dr | Ghana College of Physician and Surgeons | 2018 | 2019 |
| master's | Eugene Ahwrie | Mr | Kwame Nkrumah University of Science and Technology | 2018 | 2020 |
Memberships
| Role | Committee/board | Start Date | End Date |
|---|
Education
| Institution | Degree | Year |
|---|---|---|
| Kwame Nkrumah University of Science and Technology, Ghana | PhD | 2014-07-01 |
| Kwame Nkrumah University of Science and Technology, Ghana | MPhil | 2010-02-01 |
Areas Of Specialisation
Human Immuno-deficiency Virus (HIV) Lower Respiratory Tract Infections (LRTIs)
Grants
Publications
Authors:
Philip El-Duah
Benjamin Meyer
Augustina Sylverken
Michael Owusu
Lina Theresa Gottula
Richmond Yeboah
Jones Lamptey
Yaw Oppong Frimpong
Vitus Burimuah
Raphael Folitse
Olivia Agbenyega
Samuel Oppong
Yaw Adu-Sarkodie
Christian Drosten
Benjamin Meyer
Augustina Sylverken
Michael Owusu
Lina Theresa Gottula
Richmond Yeboah
Jones Lamptey
Yaw Oppong Frimpong
Vitus Burimuah
Raphael Folitse
Olivia Agbenyega
Samuel Oppong
Yaw Adu-Sarkodie
Christian Drosten
Date:
2019-01-11
Journal:
Viruses
Content:
Known human coronaviruses are believed to have originated in animals and made use of intermediate hosts for transmission to humans. The intermediate hosts of most of the human coronaviruses are known, but not for HCoV-NL63. This study aims to assess the possible role of some major domestic livestock species as intermediate hosts of HCoV-NL63. We developed a testing algorithm for high throughput screening of livestock sera with ELISA and confirmation with recombinant immunofluorescence assay testing for antibodies against HCoV-NL63 in livestock. Optimization of the ELISA showed a capability of the assay to significantly distinguish HCoV-NL63 from HCoV-229E (U = 27.50, p < 0.001) and HCoV-OC43 (U = 55.50, p < 0.001) in coronavirus-characterized sera. Evaluation of the assay with collected human samples showed no significant difference in mean optical density values of immunofluorescence-classified HCoV-NL63-positive and HCoV-NL63-negative samples (F (1, 215) = 0.437, p = 0.509). All the top 5% (n = 8) most reactive human samples tested by ELISA were HCoV-NL63 positive by immunofluorescence testing. In comparison, only a proportion (84%, n = 42) of the top 25% were positive by immunofluorescence testing, indicating an increased probability of the highly ELISA reactive samples testing positive by the immunofluorescence assay. None of the top 5% most ELISA reactive livestock samples were positive for HCoV-NL63-related viruses by immunofluorescence confirmation. Ghanaian domestic livestock are not likely intermediate hosts of HCoV-NL63-related coronaviruses.
Projects
Fellow:
Michael Owusu
Collaborators:
| Name | Country | Institution |
|---|---|---|
| Hubert Endz | France | Fondation Merieux |
Objectives:
1. Characterise the microbiota of the respiratory niche of HIV positive children and healthy controls children
2. Correlate specific microbiota with the respiratory disease and other laboratory markers in HIV infected children.
3. Determine the risk factors associated with exposure to specific microbiota.
Sites:
Komfo Anokye Teaching Hospital, Kumasi
Study Design:
Case-Control Study
Subjects:
Microbiology
Outcomes:
Outcomes of the study includes:
1) Knowing the aetiological agents of pneumonia in HIV infected children
2) Knowing the risk factors of respiratory disease in HIV infected children
Start Date:
2017-07-01
End Date:
2020-07-01