EDCTP Alumni Network

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All Profiles
Call Senior Fellowship (SF)
Programme EDCTP1
Start Date 2008-07-31
End Date 2010-07-30
Project Code TA.2007.40200.011
Status Completed

Title

Frequency and determinants of dual infection with different strains of HIV-1 in low- and high-risk populations in Uganda

Objectives

To evaluate the frequency and determinants of dual infection with different strains of HIV-1 in low- and high- risk populations in Uganda. This study had four major objectives and 1 minor: • Implement and validate novel sequence analysis methods developed at the Rega Institute (Belgium) for the detection of instances of co-infection and superinfection in longitudinally collected samples • Determine the clinical consequences of dual infection by comparing disease progression (viral loads and CD4+ counts) between those with and without dual infection • Determine the course of virologic recombination that occurs after dual infection • Determine the immunologic correlates of dual infection • Determine the prevalence of HIV-1 drug resistance among recently infected commercial sex workers.

Host Organisation

Institution Country
Medical Research Council Programme on AIDS - Uganda Virus Research Institute (MRC/UVRI) Uganda

Participants

Name Institution Country
Eric Arts Western Reserve University United States
Frances Gotch Imperial College London United Kingdom
Heiner Grosskurth Medical Research Council Programme on AIDS - Uganda Virus Research Institute (MRC/UVRI) Uganda
Pontiano Kaleebu Medical Research Council Programme on AIDS - Uganda Virus Research Institute (MRC/UVRI) Uganda
Philippe Lemey Katholieke Universiteit Leuven Belgium
Pietro Pala Medical Research Council Programme on AIDS - Uganda Virus Research Institute (MRC/UVRI) Uganda
Deogratius Ssemwanga Medical Research Council Programme on AIDS - Uganda Virus Research Institute (MRC/UVRI) Uganda
Annemie Vandamme Katholieke Universiteit Leuven Belgium
Carolyn Williamson University of Cape Town South Africa

Study Design

Prospective cohort study on determinants of dual infection with HIV strains

Sites

Kampala

Students Supervised

Type Name Title University Start Date End Date
Molecular Virology with Thesis Ssemwanga Deogratius PhD Makerere University 2008 2012

Results & Outcomes

Various methodologies have been optimised and used for the detection and confirmation of dual infection. The study was unable to determine the incidence of co-infection and superinfection but was able to show HIV-1 subtype distribution, multiple infections, sexual networks and partnership histories in Commercial Sex Workers in Kampala. The prevalence of transmitted drug resistance among newly infected commercial sex workers was done among 42 women that seroconverted in the high risk population. DNA sequencing work is in progress.

Publications

Ssemwanga D, Lyagoba F, Ndembi N, Mayanja BN, Larke N, Wang S, Baalwa J, Williamson C, Grosskurth H, Kaleebu P. Multiple HIV-1 infections with evidence of recombination in heterosexual partnerships in a low risk Rural Clinical Cohort in Uganda. Virology. 2011 Mar 1;411(1):113-31. Epub 2011 Jan 15.
Ndembi N, Hamers RL, Sigaloff KC, Lyagoba F, Magambo B, Nanteza B, Watera C, Kaleebu P, Rinke de Wit TF. Transmitted antiretroviral drug resistance among newly HIV-1 diagnosed young individuals in Kampala. AIDS. 2011 Apr 24;25(7):905-10
Ssemwanga D, Ndembi N, Lyagoba F, Bukenya J, Seeley J, Vandepitte J, Grosskurth H, Kaleebu P. HIV-1 subtype distribution, multiple infections, sexual networks and partnership histories in Commercial Sex Workers in Kampala, Uganda. AIDS Res Hum Retroviruses. 2012 Apr;28(4):357-65
Ssemwanga D, Ndembi N, Lyagoba F, Magambo B, Kapaata A, Bukenya J, Lubega GW, Bertagnolio S, Vandepitte J, Grosskurth H, Kaleebu P. Transmitted Antiretroviral Drug Resistance among drug-naïve Commercial Sex Workers with recent infection in Kampala, Uganda. Clin Infect Dis. 2012 May;54 Suppl 4:S339-42.

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