|Dr. Bongiwe Ndlovu
||University of KwaZulu-Natal
Objective 1: Amplification and cloning of HIV-1 Env from hyperacute, Fiebig stage I infection for the generation of pseudoviruses
Objective 2: To screen broadly neutralizing monoclonal antibodies targeting different epitopes on the Envelope glycoprotein for their ability to neutralize transmitted/founder viruses from individuals with Fiebig stage I infection, prior to initiation of combination antiretroviral therapy.
Objective 3: To characterize epitope-specific signature amino acid residues at transmission to identify immune escape pathways during primary HIV-1 infection.
University of KwaZulu-Natal
The aim of this study is to identify the best monoclonal antibodies that can be used to block transmitted/founder HIV-1 subtype C viruses from establishing infection. We plan to evaluate 9 previously well-characterized monoclonal neutralizing antibodies against 60 HIV-1 subtype C transmitted/ founder viruses generated from a well-characterized HIV-1 hyperacute FRESH cohort based in
Durban, South Africa. We will also sequence the HIV-1 envelope to identify amino acid signatures that are associated with neutralization escape. FRESH is an existing cohort of young women that are followed longitudinally, the cohort also has HIV-1 prevention awareness, career skills training and job preparedness for these young girls.
Acute HIV infected young women
TMA Career Development Fellowships