EDCTP Alumni Network

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All Profiles

Dr Nontobeko Mvubu

Call Career Development Fellowship (CDF)
Programme EDCTP2
Start Date 2021-08-01
End Date 2024-07-31
Project Code TMA2020CDF-3167
Status Active

Title

Identification and characterization of the Mycobacterium tuberculosis complex lineage-specific epigenomes, transcriptome patterns and regulatory networks and pathways

Host Organisation

Institution Country
University of KwaZulu-Natal (UKZN) South Africa

Current Organisation

University of KwaZulu-Natal

Current Job Title

Lecturer and Researcher

Students Supervised

Type Name Title University Start Date End Date
Masters Anisha Balgobin Miss University of KwaZulu-Natal 2018 2021
Masters Deepika Moti Miss University of KwaZulu-Natal 2018 2021
PhD Wadzanai Manjeese Miss University of KwaZulu-Natal 2019 2021
Masters Divenita Govender Miss University of KwaZulu-Natal 2019 2022

Education

Institution Degree Year
University of KwaZulu-Natal, South Africa PhD 2016-04-13
University of KwaZulu-Natal, South Africa BSc Honours (Microbiology) 2011-04-14
University of KwaZulu-Natal, South Africa Bachelor of Medical Science (Physiology) 2010-04-15
,

Areas Of Specialisation

Tuberculosis (TB)

Publications

Canonical pathways, networks and transcriptional factor regulation by clinical strains of Mycobacterium tuberculosis in pulmonary alveolar epithelial cells
Authors:
Mvubu, N.E.
Pillay, B.
Gamieldien, J.
Bishai, W.
Pillay, M.
Date:
2016-01-01
Journal:
Tuberculosis
Content:
Identifiers:
DOI: 10.1016/j.tube.2015.12.002
EID: 2-s2.0-84960394449
Clinical strains of Mycobacterium tuberculosis exhibit differential lipid metabolism-associated transcriptome changes in in vitro cholesterol and infection models.
Authors:
Nontobeko Eunice Mvubu , Microbiology, School of Life Sciences, College of Agriculture, Engineering and Science, University of KwaZulu-Natal, Westville
Date:
2022-12-12
Journal:
Pathogens and Disease
Content:

Many studies have identified host-derived lipids, characterised by the abundance of cholesterol, as a major source of carbon nutrition for Mycobacterium tuberculosis during infection. Members of the Mycobacterium tuberculosis complex are biologically different with regards to degree of disease, host range, pathogenicity and transmission. Therefore, the current study aimed at elucidating transcriptome changes during early infection of pulmonary epithelial cells and on an in vitro cholesterol-rich minimal media, in M. tuberculosis clinical strains F15/LAM4/KZN and Beijing, and the laboratory H37Rv strain. Infection of pulmonary epithelial cells elicited the upregulation of fadD28 and hsaC in both the F15/LAM4/KZN and Beijing strains and the downregulation of several other lipid-associated genes. Growth curve analysis revealed F15/LAM4/KZN and Beijing to be slow growers in 7H9 medium and cholesterol-supplemented media. RNA-seq analysis revealed strain-specific transcriptomic changes, thereby affecting different metabolic processes in an in vitro cholesterol model. The differential expression of these genes suggests that the genetically diverse M. tuberculosis clinical strains exhibit strain-specific behaviour that may influence their ability to metabolise lipids, specifically cholesterol, which may account for phenotypic differences observed during infection.

Identifiers:
Funded by HTTPS://DOI.ORG/10.1093/FEMSPD/FTAC046: not informed
Lineage-specific differences in lipid metabolism and its impact on clinical strains of Mycobacterium tuberculosis
Authors:
Date:
2020-09-01
Journal:
Microbial Pathogenesis
Content:
Identifiers:
DOI: 10.1016/j.micpath.2020.104250
Part of ISSN: 0882-4010
Infection of pulmonary epithelial cells by clinical strains of M. tuberculosis induces alternate splicing events
Authors:
Date:
2020-08-01
Journal:
Gene
Content:
Identifiers:
DOI: 10.1016/j.gene.2020.144755
Part of ISSN: 0378-1119
Exploring the Use of Medicinal Plants and Their Bioactive Derivatives as Alveolar NLRP3 Inflammasome Regulators during Mycobacterium tuberculosis Infection
Authors:
Nontobeko E. Mvubu , author
Thamsanqa E. Chiliza , author
Date:
2021-08-31
Journal:
International Journal of Molecular Sciences
Content:
Identifiers:
DOI: 10.3390/ijms22179497
Mycobacterium tuberculosis strains induce strain-specific cytokine and chemokine response in pulmonary epithelial cells
Authors:
Mvubu, N.E.
Pillay, B.
McKinnon, L.R.
Pillay, M.
Date:
2017-01-01
Journal:
Cytokine
Content:
Identifiers:
DOI: 10.1016/j.cyto.2017.09.027
EID: 2-s2.0-85030657784
Mycobacterium tuberculosis-Induced Maternal Immune Activation Promotes Autism-Like Phenotype in Infected Mice Offspring
Authors:
Wadzanai Manjeese , author
Nontobeko E. Mvubu , author
Adrie J. C. Steyn , author
Thabisile Mpofana , author
Date:
2021-04-23
Journal:
International Journal of Environmental Research and Public Health
Content:
Identifiers:
DOI: 10.3390/ijerph18094513
M ycobacterium tuberculosis causes a leaky blood‐brain barrier and neuroinflammation in the prefrontal cortex and cerebellum regions of infected mice offspring
Authors:
Wadzanai Manjeese , author
Nontobeko.E. Mvubu , author
Adrie.J.C. Steyn , author
Thabisile Mpofana , author
Date:
2021-05-01
Journal:
International Journal of Developmental Neuroscience
Content:
Identifiers:
DOI: 10.1002/jdn.10116
Mycobacterium tuberculosis strains exhibit differential and strain-specific molecular signatures in pulmonary epithelial cells
Authors:
Mvubu, N.E.
Pillay, B.
Gamieldien, J.
Bishai, W.
Pillay, M.
Date:
2016-01-01
Journal:
Developmental and Comparative Immunology
Content:
Identifiers:
DOI: 10.1016/j.dci.2016.07.022
EID: 2-s2.0-84982165931

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