EDCTP Alumni Network

Fostering excellence and collaboration in the next generation of researchers

Call Career Development Fellowship (CDF)
Programme EDCTP2
Start Date 2021-01-01
End Date 2023-12-31
Project Code TMA2019CDF-2662
Status Active

Title

Predictors of local emergence and spread of Artemisinin resistance among Ugandan Plasmodium falciparum parasites (Pfkelch13 emergence)

Host Organisation

Institution Country
Makerere University Uganda

Current Organisation

Makerere University, College of Health Sciences

Current Job Title

Lecturer

Awards

2021 MaKRIF

Students Supervised

Type Name Title University Start Date End Date
PhD Hindum Lanyero Assistant lecturer Makerere University, College of Health Science 2018 2022
PhD Kimbowa Isaac Assistant lecturer Makerere University, College of Health Science 2018 2022

Education

Institution Degree Year
Makerere University, Uganda Bachelor of Science 2003-08-23
Makerere University, Uganda Master of Science in Pharmacology 2011-02-12
Makerere University, Uganda Philosophy 2017-02-12

Areas Of Specialisation

Malaria

Publications

Authors:
Ocan M , author
Akena D , author
Nsobya S , author
Kamya MR , author
Senono R , author
Kinengyere AA , author
Obuku E , author
Date:
2018-11-01
Journal:
Systematic reviews
Content:
Identifiers:
Authors:
Moses Ocan , author
Brenda Allen Kawala , author
Ephraim Kisangala , author
Regina Ndagire , author
Rachel Nante Wangi , author
Daniel Semakula , author
Alison Annet Kinengyere , author
Nelson K. Sewankambo , author
Thomas Katairo , author
Allen Eva Okullo , author
Fred Nalugoda , author
Eve Namisango , author
Kevin Ojiambo Ouma , author
Robert Apunyo , author
Loyce Nakalembe , author
Ash Luwambo , author
Ekwaro Anthony Obuku , author
Date:
2021-09-27
Journal:
Content:
Identifiers:
Authors:
Ocan M , author
Bbosa GS , author
Waako P , author
Ogwal-Okeng J , author
Obua C , author
Date:
2014-06-01
Journal:
BMC public health
Content:
Identifiers:
Authors:
Ocan M , author
Bwanga F , author
Bbosa GS , author
Bagenda D , author
Waako P , author
Ogwal-Okeng J , author
Obua C , author
Date:
2014-03-01
Journal:
PloS one
Content:
Identifiers:
Authors:
Madut NA , author
Ocan M , author
Muwonge A , author
Muma JB , author
Nasinyama GW , author
Godfroid J , author
Jubara AS , author
Kankya C , author
Date:
2019-05-01
Journal:
BMC infectious diseases
Content:
Identifiers:
Authors:
Lanyero H , author
Eriksen J , author
Obua C , author
Stålsby Lundborg C , author
Nanzigu S , author
Katureebe A , author
Kalyango JN , author
Ocan M , author
Date:
2020-06-01
Journal:
PloS one
Content:
Identifiers:
Authors:
Rukundo GZ , author
Kemigisha E , author
Ocan M , author
Adriko W , author
Akena DH , author
Date:
2018-12-01
Journal:
Systematic reviews
Content:
Identifiers:
Authors:
Ocan M , author
Akena D , author
Nsobya S , author
Kamya MR , author
Senono R , author
Kinengyere AA , author
Obuku EA , author
Date:
2018-07-01
Journal:
Systematic reviews
Content:
Identifiers:
Authors:
Wakida EK , author
Ocan M , author
Rukundo GZ , author
Sebutinde P , author
Maling S , author
Okello ES , author
Talib ZM , author
Obua C , author
Date:
2021-03-01
Journal:
Content:
Identifiers:
Authors:
Moses Ocan , author
Brenda Allen Kawala , author
Ephraim Kisangala , author
Regina Ndagire , author
Rachel Nante Nante Wangi , author
Daniel Semakula , author
Alison Annet Kinengyere , author
Nelson K. Sewankambo , author
Thomas Katairo , author
Allen Eva Okullo , author
Fred Nalugoda , author
Eve Namisango , author
Kevin Ojiambo Ouma , author
Robert Apunyo , author
Loyce Nakalembe , author
Ash Luwambo , author
Ekwaro Anthony Obuku , author
Date:
2021-02-10
Journal:
Content:
Identifiers:
Authors:
Moses Ocan , Ocan et al. BMC Infectious Diseases (2016) 16:428
Date:
2016-08-19
Journal:
Malaria Journal
Content:

Background: In the absence of an effective vaccine, malaria treatment and eradication is still a challenge in most
endemic areas globally. This is especially the case with the current reported emergence of resistance to artemisinin
agents in Southeast Asia. This study therefore explored the prevalence of K13-propeller gene polymorphisms among
Plasmodium falciparum parasites in northern Uganda.
Methods: Adult patients (≥18 years) presenting to out-patients department of Lira and Gulu regional referral hospitals
in northern Uganda were randomly recruited. Laboratory investigation for presence of plasmodium infection among
patients was done using Plasmodium falciparum exclusive rapid diagnostic test, histidine rich protein-2 (HRP2) (Pf).
Finger prick capillary blood from patients with a positive malaria test was spotted on a filter paper Whatman no. 903.
The parasite DNA was extracted using chelex resin method and sequenced for mutations in K13-propeller gene using
Sanger sequencing. PCR DNA sequence products were analyzed using in DNAsp 5.10.01software, data was further
processed in Excel spreadsheet 2007.
Results: A total of 60 parasite DNA samples were sequenced. Polymorphisms in the K13-propeller gene were detected
in four (4) of the 60 parasite DNA samples sequenced. A non-synonymous polymorphism at codon 533 previously
detected in Cambodia was found in the parasite DNA samples analyzed. Polymorphisms at codon 522 (non-synonymous)
and codon 509 (synonymous) were also found in the samples analyzed. The study found evidence of positive selection in
the Plasmodium falciparum population in northern Uganda (Tajima’s D=−1.83205; Fu and Li’s D=−1.82458).
Conclusions: Polymorphism in the K13-propeller gene previously reported in Cambodia has been found in the Ugandan
Plasmodium falciparum parasites. There is need for continuous surveillance for artemisinin resistance gene markers in
the country.

Identifiers:
Authors:
Moses Ocan , author
Dickens Akena , author
Sam Nsobya , author
Moses R. Kamya , author
Richard Senono , author
Alison Annet Kinengyere , author
Ekwaro A. Obuku , author
Date:
2019-12-01
Journal:
Malaria Journal
Content:
Identifiers:
Authors:
Obuku EA , author
Lavis JN , author
Kinengyere A , author
Ssenono R , author
Ocan M , author
Mafigiri DK , author
Ssengooba F , author
Karamagi C , author
Sewankambo NK , author
Date:
2018-08-01
Journal:
Health research policy and systems
Content:
Identifiers:
Authors:
Ocan M , author
Bwanga F , author
Okeng A , author
Katabazi F , author
Kigozi E , author
Kyobe S , author
Ogwal-Okeng J , author
Obua C , author
Date:
2016-08-01
Journal:
BMC infectious diseases
Content:
Identifiers:
Authors:
Ocan M , author
Aono M , author
Bukirwa C , author
Luyinda E , author
Ochwo C , author
Nsambu E , author
Namugonza S , author
Makoba J , author
Kandaruku E , author
Muyende H , author
Nakawunde A , author
Date:
2017-09-01
Journal:
BMC public health
Content:
Identifiers:
Authors:
Madut NA , author
Nasinyama GW , author
Muma JB , author
Sube KLL , author
Ocan M , author
Muwonge A , author
Godfroid J , author
Jubara AS , author
Kankya C , author
Date:
2018-06-01
Journal:
PloS one
Content:
Identifiers:
Authors:
Lyus R , author
Pollock A , author
Ocan M , author
Brhlikova P , author
Date:
2020-06-01
Journal:
Bulletin of the World Health Organization
Content:
Identifiers:
Authors:
Ocan M , author
Obuku EA , author
Bwanga F , author
Akena D , author
Richard S , author
Ogwal-Okeng J , author
Obua C , author
Date:
2015-08-01
Journal:
BMC public health
Content:
Identifiers:
Authors:
Ocan M , author
Manabe YC , author
Baluku H , author
Atukwase E , author
Ogwal-Okeng J , author
Obua C , author
Date:
2015-09-01
Journal:
BMC pharmacology & toxicology
Content:
Identifiers:
Authors:
Inyimai SP , author
Ocan M , author
Wabwire B , author
Olupot-Olupot P , author
Date:
2018-07-01
Journal:
Journal of tropical medicine
Content:
Identifiers:
Authors:
Moses Ocan , author
Dickens Akena , author
Sam Nsobya , author
Moses R. Kamya , author
Richard Senono , author
Alison Annet Kinengyere , author
Ekwaro Obuku , author
Date:
2019-12-01
Journal:
Malaria Journal
Content:
Identifiers:

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