EDCTP Alumni Network

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Call EDCTP-AREF Preparatory Fellowships (PF)
Programme EDCTP2
Start Date 2021-03-01
End Date 2023-01-31
Project Code TMA2019PF-2694
Status Active

Title

Use of an Aedes Salivary Biomarker to Assess Arboviral disease Transmission Risk in Northern Tanzania

Objectives

To determine human exposure to Aedes mosquitoes as measured by specific anti-IgG antibodies against Aedes Nterm-34kDa salivary peptide, and to assess whether level of antibodies could be useful in assessing the risk of dengue, Zika and chikungunya transmission and evaluate efficacy of vector control strategies in North-Eastern Tanzania.

Host Organisation

Institution Country
Centre for Research in Infectious Diseases (CRID), Yaoundé, Cameroon

Participants

Name Institution Country
Prof. Reginald Kavishe Kilimanjaro Christian Medical University College (KCMUCo) Tanzania, United Republic of
Prof. Blandina Mmbaga Kilimanjaro Clinical Research Institute Tanzania, United Republic of
Dr. Emmanuel Elanga N'dille Centre for Research in Infectious Diseases (CRID), Yaoundé, Cameroon

Study Design

The study will be conducted in three villages in Bondo site, Tanga. Surveys will be conducted three Vector control will be performed few days after the T1 visit. A total of 362 participants will be enrolled.. Participants will be followed longitudinally to screen level of ant- IgG antibody response against Aedes salivary gland peptides

Sites

Bondo site, Tanga

Phd Study

Title University Start Date End Date
Dengue and Chikungunya infections: Prevalence and Risk Factors Among Community Members in North Eastern Tanzania Kilimanjaro Christian Medical University College (KCMUCo) 2014-02-03 2017-11-18

Students Supervised

Type Name Title University Start Date End Date
MSc Anna Kaaya Assessing the Risk of Malaria and Arboviral Transmission by Evaluating Mosquito vector Infectivity and Measuring Serological Biomarkers in Bondo, Tanga Kilimanjaro Christian Medical University College (KCMUCo) 2021 2022
MSc Emmanuel Mkumbo Association between severe plasmodium malaria and expression of HRP-2 (Histidine Rich Protein 2) among villagers of Bondo site, Handeni district, Tanga region, Tanzania. Kilimanjaro Christian Medical University College (KCMUCo) 2021 2022
MSc Francis Mponela Prevalence, associated factors and spatial distribution of dengue and chikungunya virus among residents of Handeni District in Tanga Kilimanjaro Christian Medical University College (KCMUCo) 2021 2022

Results & Outcomes

A total of 1.3 % (5/138) had recent dengue infection. Active chikungunya infection was confirmed by polymerase chain reaction for 4.2% (11/263) of the participants. Headache and joint pain were significant predictors of chikungunya infection (Measured by IgM sero-positivity) with OR=3.03 (2.06 – 4.40), p<0.01 and OR = 1.80 (1.25 – 2.59), p<0.01, respectively. Risk factors associated with chikungunya IgM sero-positivity were environmental factors such as presence of uncovered containers/car-tire in the surrounding area of the household, keeping hoofed animal and vegetation (<100m). The study found a large cluster of chikungunya seropositive cases in Rundugai village (RR=2.58, p=0.01). The study found that 15.2% (19/125) of the community members and 53.6% (67/125) of the healthcare workers had good knowledge score for dengue.

Publications

Kajeguka, D.C., Kaaya, R.D., Mwakalinga, S. et al. Prevalence of dengue and chikungunya virus infections in north-eastern Tanzania: a cross sectional study among participants presenting with malaria-like symptoms. BMC Infect Dis 16, 183 (2016). https://doi.org/10.1186/s12879-016-1511-5
doi:10.1111/tmi.12863
Homenauth E, Kajeguka D, Kulkarni MA. J Epidemiol Community Health Published Online First: [please include Day Month Year]. doi:10.1136/jech- 2017-209119
DOI:https://doi.org/10.1016/j.idh.2017.04.005
Doi: http://dx.doi.org/10.4314/thrb.v19i4.1
Saringe, S., Kajeguka, D.C., Kagirwa, D.D. et al. Healthcare workers knowledge and diagnostic practices: a need for dengue and chikungunya training in Moshi Municipality, Kilimanjaro Tanzania. BMC Res Notes 12, 43 (2019). https://doi.org/10.1186/s13104-019-4074-x

Current Organisation

Kilimanjaro Christian Medical University College (KCMUCo)

Current Job Title

Lecturer

Students Supervised

Type Name Title University Start Date End Date
Debora Charles Kajeguka

Publications

Authors:
Robert D. Kaaya, Debora C. Kajeguka, Johnson J. Matowo, Arnold J. Ndaro, Franklin W. Mosha, Jaffu O. Chilongola Reginald A. Kavishe , Kaaya, R.D., Kajeguka, D.C., Matowo, J.J. et al. Predictive markers of transmission in areas with different malaria endemicity in north-eastern Tanzania based on seroprevalence of antibodies against Plasmodium falciparum. BMC Res Notes 14, 404 (2021). https://doi.org/10.1186/s13104-021-05818-y
Date:
2021-11-01
Journal:
BMC Research Notes
Content:

Objective

A community-based cross-sectional study was done to assess Plasmodium falciparum exposure in areas with different malaria endemicity in north-eastern Tanzania using serological markers; PfAMA-1 and PfMSP-119.

Results

Bondo had a higher seroprevalence 36.6% (188) for PfAMA-1 as compared to Hai 13.8% (33), χ2 = 34.66, p < 0.01. Likewise, Bondo had a higher seroprevalence 201(36.6%) for PfMSP-1 as compared to Hai 41 (17.2%), χ2 = 29.62, p < 0.01. Anti-PfAMA-1 titters were higher in malaria positive individuals (n = 47) than in malaria negative individuals (n = 741) (p = 0.07). Anti-PfMSP-1 antibody concentrations were significantly higher in malaria-positive individuals (n = 47) than in malaria-negative individuals (n = 741) (p = 0.003). Antibody response against PfAMA-1 was significantly different between the three age groups; < 5 years, 5 to 15 years and > 15 years in both sites of Bondo and Hai. Likewise, antibody response against PfMSP-119 was significantly different between the three age groups in the two sites (p < 0.001). We also found significant differences in the anti-PfAMA-1and anti-PfMSP-119 antibody concentrations among the three age groups in the two sites (p = 0.004 and 0.005) respectively. Immunological indicators of P. falciparum exposure have proven to be useful in explaining long-term changes in the transmission dynamics, especially in low transmission settings.

 

Identifiers:
Not Informed: not informed
Authors:
Date:
2017-03-01
Journal:
6th East Africa Health and Scientific Conference
Content:
Identifiers:

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