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All Profiles
Call Career Development Fellowship (CDF)
Programme EDCTP2
Start Date 2017-06-01
End Date 2020-05-31
Project Code TMA2015CDF979
Status Active

Title

Rapid detection of Mycobaterium ulcerans infection by recombinase polymerase amplification

Objectives

This study is to explore the potential of using recombinase polymerase amplification (RPA) as a tool for Buruli ulcer diagnosis. RPA has emerged as a novel isothermal technology for use in molecular diagnosis of infectious diseases. This means that it can be carried out at room temperature in unsophisticated laboratories in West Africa close to patients. The key goal is to develop a diagnostic test for the early detection of Buruli ulcer in symptomatic patients with sufficient positive predictive value to put patients on appropriate treatment.

Host Organisation

Institution Country
Kwame Nkrumah University of Science and Technology Ghana

Study Design

Cross-sectional study

Sites

Ashanti Akim North
Ahafo Ano North
Upper Denkyira Districts

Phd Study

Title University Start Date End Date
Cellular immune response to mycobacterium ulcerans infection Kwame Nkrumah University of Science and Technology 2011-08-01 2015-06-01

Students Supervised

Type Name Title University Start Date End Date
Mphil Hubert S. Ahor Mr Kwame Nkrumah University of Science and Technology 2017 2019
Mphil Louis Kyei-Tuffour Mr Kwame Nkrumah University of Science and Technology 2018 2020

Results & Outcomes

Ongoing

Publications

Simpson H et al. Mapping the global distribution of Buruli ulcer: a systematic review with evidence consensus. Lancet Glob Health. 2019 Jul;7(7):e912-e922. doi: 10.1016/S2214-109X(19)30171-8
Frimpong M et al. Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay. PLoS Negl Trop Dis. 2019 Feb 1;13(2):e0007155. doi: 10.1371/journal.pntd.0007155
Frimpong, Michael, et al. "Paradoxical reactions in Buruli ulcer after initiation of antibiotic therapy: Relationship to bacterial load." PLoS neglected tropical diseases 13.8 (2019): e0007689
Frimpong, M.; Ahor, H.S.; Sakyi, S.A.; Agbavor, B.; Akowuah, E.; Phillips, R.O. Rapid Extraction Method of Mycobacterium ulcerans DNA from Clinical Samples of Suspected Buruli Ulcer Patients. Diagnostics 2019, 9, 204
Amoako, Y.A., Frimpong, M., Awuah, D.O. et al. Providing insight into the incubation period of Mycobacterium ulcerans disease: two case reports. J Med Case Reports 13, 218 (2019). https://doi.org/10.1186/s13256-019-2144-2

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