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Call Career Development Fellowship (CDF)
Programme EDCTP2
Start Date 2017-08-01
End Date 2020-07-01
Project Code TMA2015CDF995
Status Active

Title

Urinary Cytokine ELISA: A tool to monitor urinary tract pathology in Schistosoma haematobium infections

Objectives

To assess urinary IL-6 and IL-10 ELISA, as a tool for assessing S. haematobium-related urinary tract pathology before and after treatment in a S. haematobium endemic community of Kenya using a commercially available cytokine ELISA kit.

Host Organisation

Institution Country
Universiy of Nairobi Kenya

Participants

Name Institution Country
Kariuki Njaanake University of Nairobi Kenya

Study Design

Diagnostic study

Sites

Kwale County

Students Supervised

Type Name Title University Start Date End Date
MSc. Fiona Onyango University of Nairobi
MSc. Job Omondi University of Nairobi

Publications

Projects

Fellow:
Humphrey Kariuki Njaanake
Collaborators:
Name Country Institution
H. Kariuki Njaanake Kenya University of Nairobi
Objectives:
General Objective To assess urinary IL-6 and IL-10 ELISA, as a tool for assessing S. haematobium-related urinary tract pathology before and after treatment in a S. haematobium endemic community in Machakos County of Kenya using a commercially available cytokine ELISA kit Specific Objective 1. To correlate levels of urinary IL-6 and IL-10 to S. haematobium-related pathology by comparing levels of urinary IL-6 and IL-10 in schoolchildren with and without ultrasound-detectable pathology 2. To assess levels of urinary IL-6 and IL- 10 in relation to age in S. haematobium-infected children by comparing the levels between infected and non-infected children 3. To compare changes in urinary IL-6, IL-10 and ECP levels before and after treatment by assessing the levels of IL-6, IL-10 and ECP in urine from same S. haematobium-infected children before and after two annual treatments with praziquantel 1. To determine the rate of degradation of urinary IL-6 and IL-10 at selected temperature ranges by comparing the level of the cytokines in urine samples stored at -20 °C, 4 °C and room temperature (25 °C) before use in IL-6 and IL-10 ELISA.
Sites:
Kwale County
Study Design:
Longitudinal
Subjects:
Primary School Children
Outcomes:
Urinary cytokine levels in children
Start Date:
2017-09-01
End Date:
2020-10-05

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