Fellow:
Humphrey Kariuki Njaanake
Collaborators:
Name |
Country |
Institution
|
H. Kariuki Njaanake |
Kenya |
University of Nairobi |
Objectives:
General Objective
To assess urinary IL-6 and IL-10 ELISA, as a tool for assessing S. haematobium-related urinary tract pathology
before and after treatment in a S. haematobium endemic community in Machakos County of Kenya using a commercially available cytokine ELISA kit
Specific Objective
1. To correlate levels of urinary IL-6 and IL-10 to S. haematobium-related pathology by comparing levels of urinary IL-6 and IL-10 in schoolchildren with and without ultrasound-detectable pathology
2. To assess levels of urinary IL-6 and IL- 10 in relation to age in S. haematobium-infected children by comparing the levels between infected and non-infected children
3. To compare changes in urinary IL-6, IL-10 and ECP levels before and after treatment by assessing the levels of IL-6, IL-10 and ECP in urine from same S. haematobium-infected children before and after two annual treatments with praziquantel
1. To determine the rate of degradation of urinary IL-6 and IL-10 at selected temperature ranges by comparing the level of the cytokines in urine samples stored at -20 °C, 4 °C and room temperature (25 °C) before use in IL-6 and IL-10 ELISA.
Study Design:
Longitudinal
Subjects:
Primary School Children
Outcomes:
Urinary cytokine levels in children