Dr Humphrey Kariuki Njaanake
| Call | Career Development Fellowship (CDF) |
| Programme | EDCTP2 |
| Start Date | 2017-08-01 |
| End Date | 2020-07-01 |
| Project Code | TMA2015CDF995 |
| Status | Completed |
Title
Urinary Cytokine ELISA: A tool to monitor urinary tract pathology in Schistosoma haematobium infections
Objectives
To assess urinary IL-6 and IL-10 ELISA, as a tool for assessing S. haematobium-related urinary tract pathology before and after treatment in a S. haematobium endemic community of Kenya using a commercially available cytokine ELISA kit.
Host Organisation
| Institution | Country |
|---|---|
| Universiy of Nairobi | Kenya |
Participants
| Name | Institution | Country |
|---|---|---|
| Kariuki Njaanake | University of Nairobi | Kenya |
Study Design
Diagnostic study
Sites
| Kwale County |
Students Supervised
| Type | Name | Title | University | Start Date | End Date |
|---|---|---|---|---|---|
| MSc. | Fiona Onyango | University of Nairobi | |||
| MSc. | Job Omondi | University of Nairobi |
Publications
Projects
Fellow:
Humphrey Kariuki Njaanake
Collaborators:
| Name | Country | Institution |
|---|---|---|
| H. Kariuki Njaanake | Kenya | University of Nairobi |
Objectives:
General Objective
To assess urinary IL-6 and IL-10 ELISA, as a tool for assessing S. haematobium-related urinary tract pathology
before and after treatment in a S. haematobium endemic community in Machakos County of Kenya using a commercially available cytokine ELISA kit
Specific Objective
1. To correlate levels of urinary IL-6 and IL-10 to S. haematobium-related pathology by comparing levels of urinary IL-6 and IL-10 in schoolchildren with and without ultrasound-detectable pathology
2. To assess levels of urinary IL-6 and IL- 10 in relation to age in S. haematobium-infected children by comparing the levels between infected and non-infected children
3. To compare changes in urinary IL-6, IL-10 and ECP levels before and after treatment by assessing the levels of IL-6, IL-10 and ECP in urine from same S. haematobium-infected children before and after two annual treatments with praziquantel
1. To determine the rate of degradation of urinary IL-6 and IL-10 at selected temperature ranges by comparing the level of the cytokines in urine samples stored at -20 °C, 4 °C and room temperature (25 °C) before use in IL-6 and IL-10 ELISA.
Sites:
Kwale County
Study Design:
Longitudinal
Subjects:
Primary School Children
Outcomes:
Urinary cytokine levels in children
Start Date:
2017-09-01
End Date:
2020-10-05