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Call Career Development Fellowship (CDF)
Programme EDCTP2
Start Date 2017-08-01
End Date 2020-07-01
Project Code TMA2015CDF995
Status Active

Title

Urinary Cytokine ELISA: A tool to monitor urinary tract pathology in Schistosoma haematobium infections

Objectives

To assess urinary IL-6 and IL-10 ELISA, as a tool for assessing S. haematobium-related urinary tract pathology before and after treatment in a S. haematobium endemic community of Kenya using a commercially available cytokine ELISA kit.

Host Organisation

Institution Country
Universiy of Nairobi Kenya

Study Design

Diagnostic study

Publications

Projects

Fellow:
Humphrey Kariuki Njaanake
Collaborators:
Name Country Institution
H. Kariuki Njaanake Kenya University of Nairobi
Objectives:
General Objective To assess urinary IL-6 and IL-10 ELISA, as a tool for assessing S. haematobium-related urinary tract pathology before and after treatment in a S. haematobium endemic community in Machakos County of Kenya using a commercially available cytokine ELISA kit Specific Objective 1. To correlate levels of urinary IL-6 and IL-10 to S. haematobium-related pathology by comparing levels of urinary IL-6 and IL-10 in schoolchildren with and without ultrasound-detectable pathology 2. To assess levels of urinary IL-6 and IL- 10 in relation to age in S. haematobium-infected children by comparing the levels between infected and non-infected children 3. To compare changes in urinary IL-6, IL-10 and ECP levels before and after treatment by assessing the levels of IL-6, IL-10 and ECP in urine from same S. haematobium-infected children before and after two annual treatments with praziquantel 1. To determine the rate of degradation of urinary IL-6 and IL-10 at selected temperature ranges by comparing the level of the cytokines in urine samples stored at -20 °C, 4 °C and room temperature (25 °C) before use in IL-6 and IL-10 ELISA.
Sites:
Kwale County
Study Design:
Longitudinal
Subjects:
Primary School Children
Outcomes:
Urinary cytokine levels in children
Start Date:
2017-09-01
End Date:
2020-10-05

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